服務熱線
15201163601
-
詳細解析！et404毒素試劑盒的檢測原理和步驟
發(fā)布時間： 2023-02-26 點擊次數(shù)： 921次et404毒素試劑盒主要是用來檢測毒素的一種經(jīng)濟、快捷的檢測初篩工具，現(xiàn)在當前市面上主要有毒素B1試劑盒、毒素M1試劑盒、毒素總量試劑盒。它的檢測原理：et404毒素試劑盒采用競爭ELISA，在微孔板上預包被毒素抗原，加入樣本/毒素總量標準品溶液及辣根過氧化物酶標記的毒素總量抗體。樣本或標準品溶液中的毒素與預包被在微孔板上的毒素抗原競爭結(jié)合辣根過氧化物酶標記的毒素總量抗體。未結(jié)合的酶標抗體在洗滌時被除去，再加入TMB顯色液，讀取吸光值。樣本的吸光值與其所含殘留物毒素總量的含量成負相關。對照標準曲線，即可得出相應殘留物毒素總量的含量。et404毒素試劑盒的檢測步驟：樣品處理：取1ml樣品置于5ml離心管中，向離心管中加2ml提取劑，振搖2～3min后，用離心機以2000r/min，離心5min，取2ml上清液至另一個5ml離心管中，向其中加2ml凈化劑，振搖5min，用離心機以2000r/min離心5min，吸取全部下層至點滴板的一個孔中自然揮干（或加熱將其揮干），備用；樣品檢測：向點滴板上滴加4滴緩沖液，使其充分溶解揮干后的殘留物；取出檢測卡，并做好標記，用袋中吸管吸取點滴板上的全部樣液，滴加到檢測卡的加樣孔中（逐滴滴下），5～10min內(nèi)觀察結(jié)果；結(jié)果判斷：陰性：測試線(T)與對照線(C)都出現(xiàn)，表明樣品中毒素的濃度小于0.5μg/kg。陽性：只有對照線(C)，無測試線(T)，表明樣品中毒素的濃度大于或等于0.5μg/kg。無效：對照線(C)和測試線(T)都不出現(xiàn)，或者對照線（C）不出現(xiàn)。
產(chǎn)品中心
Products
-
化學試劑
Origene parkell pan-biotech PAN lexogen Lampire labtidebiotech laborator labome ABI enzolifesciences emsdiasum EtonBioscience Equitech-bio kirgen invivogen integralmolecular Instech IndoorBiotechnologies INCELL IMMUSMOL G-Biosciences IMMUNOLOGICALS idtdna IMMUNODX ILLUMINA Immunodiagnostics IBALifeSciences Hypoxyprobe Horizon Cygnus Technologies Hellobio Greiner Cell Signaling Glycobiotech Corning FST Evrogen eurofins BioFire Defense biodatacorp atlasantibodies ATCC Astartebio ArcticZymes aobious anshlabs Advanced ChemTech Advaced Cell Diagnostics AdvanDx Agar Scientific ACBR TRc polyscience Fluka Gene Bridges fortebio Biocrick Fisher bioflux biomax beckman AmericanElements MOLTOX Permagen USA Scientific Chemgenes寡核苷酸 Asuragen vmrd抗體 Thermo asusragen AmplideX toxin polyplus signagen Berry&Associates ALZET滲透泵 alzet abcr abvector SGI-DNA Goldbio Iduron Sgidna Chromsystems Coriell GE Chemgenes Matreya Streck VMRD Sigma Cerilliant Innovative Cell Technologies Diagenode Trilinkbiotech Medkoo life diagnostics Gibco Neuromab全國總代 Novus BioLegend Bethyl Toxin Technology Listlabs Seracare 寶利嘉 賽默飛 安迪生物 行業(yè)專用試劑
-
生物試劑
Biosharp Sciex Seahorse Proteintech DiaMag complementtech PeproTech hooke Nzytech Pierce pepnet pelfreez PALL ozbiosciences OXOID ORFLO ommscientific omega nanopore ncbi NexcelomBioscience Mpbio MultiSciences MyBioSource Musechem MiltenyiBiotec minerva-biolabs microspheres-nanospheres microfil microbix modiquestresearch MOBITEC mercodia merckmillipore medicago JPT kerafast Kangchen Malvern magen Kapa Macrocyclics Macherey mabtech Lucigen leica Lifespan lifesensors lifeline life-ilab Lifecore life Licor advancedchemtech Affinity Abgent Abmgood ActiveMotif Acros Acrobiosystems enzymatics epicypher Elastin elabscience Echelon eBioscience eastcoast eaglebio E&K SCIENTIFIC ekfusa duran DSHB Dharmacon DGRC Dako Cypex comptech Encorbio Jetbiofil JCRBcellbank ISOSCIENCES INVIVOCHEM iris-biotech Full Moon BioSystems GBI FormnMax Finescience Favorgen CordenPharma CIL Chromadex Clodronateliposomes Chromogenix Chiron chemservice cellvis cellutron CELLTREAT cellscript cellbiolabs cellecta cedarlane cdnisotopes carbosynth cambio calbioreagents Buchem Boston Cellron Bioworld Biowest Biotium biostatus BiosPacific Biosearch BIOplastics Biooscientific BioPAL Biomol BIOMED Biomatik bioline Biocoat Bioclone Biocision biochain華新康信 biocell Biocare BIAseparations BDpharmingen Baseclick Badrilla B2S Life Sciences Axon Medchem AXIS-SHIELD avivasysbio avidity AVES-LABS Anatrace Americanbio AlphaDiagnoesticInternational Applied Genetics Laboratories Alstem Alomone AcceGen Akina AdvancedBioMatriX Adipogen Accuratechemical Academybiomed Abcam Zymo Research Addexbio ABL Abbexa Aablocks ZeptoMetrix Tocris TAKARA Spherotech Santa Roche Nist PBL MRC Holland rd-biotech Cellgs LC-LAB Greatcell Energy Hyclone Greerlabs GlycoSyn GlycoTech EY laboratories Cayman CanSyn Bioxcell Bio－Rad Agdia Abclonal amsbio auditmicro avantilipids Alsachim HTDialysis jetPRIME Carna Biosciences FItzgerald